Abstract:Patrinia Villosa(P.V.) can cure diarrhea, but it can also do good to constipation. Not only the leaves and the stems can relax the constipation, but also the water solution extracted from P.V. has the similar function. In order to know the effective ingredients to prevent and cure constipation extracted in the water solution, the extraction technology of soluble polysaccharides from wild P.V. was studied, and single factor test and orthogonal experiment design methods (L9(33)) were applied to analyze the influence of each factor in solid liquid ratio, temperature and time on the extraction percent of the polysaccharides from the water solution of P.V.. Experimental results indicated that temperature and solid liquid ratio significantly affect the extraction percent of the soluble polysaccharides. The optimum solid liquid ratio is 1∶20, temperature is 100℃ and the extraction time is 6 hours. In this condition, the extraction percent of soluble polysaccharides is 2.58%.