In order to study the storage technology of Ginkgo biloba seed at normal atmospheric temperature, the effects of six kinds of pre-storage methods on the quality and physiological characteristics of stored Ginkgo biloba seeds were studied. The results show that the effect of treatment by immersing the seeds in NaHSO₃ solution with 10 g/L for 1 hour,and then storing at 25℃ for 30 days is the best. The water loss, hardening rate, rottening rate, length of embryo and germination capacity of the seeds treated are 1.02%, 0.52%, 1.36%, 3.5 mm and 92.57%, respectively, while 89.14% of rottening rate and only 6.53% of germination capacity from CK. On the other hand, two chemicals, Sporgon and Imazalil were selected by inhibition zone method as inhibitors towards main pathogenic fungi in post-harvest Ginkgo biloba seeds. Their optimal concentrations were 0.8 g/L and 1.0 g/L, respectively, and their obvious complementarity of inhibiting effect was observed. It was found that when the seeds were treated with mixing 0.8 g/L of Sporgon with 1.0 g/L of Imazalil for 30 minutes and then packed in 0.02 mm thick polyethylene bag at 20℃ for 90 days, the rottening rate was only 2.51%, which was much lower than that treated with one of them alone, but the germination capacity was still 91.24%, hardening rate, water loss and length of embryo were 2.56%, 3.94% and 4.36 mm, respectively. The results also indicated that the other physiological characteristics were all decreasing except for increasing by 37.08% of the lipase activity after the seeds were treated with mixed fungus inhibitor. The respiration intensity (RI) was most significantly decreasing by 69.92%. The vitality of Ginkgo biloba seeds was still strong although the RI of the seeds was inhibited remarkably, and catalase(CAT), pectinesterase(PE), polygalacturonase(PG) were slightly decreasing.