Solution culture experiment was carried out to study the growth, activity of preoxiodse(POD), proline content, root system vigor, and Zn uptake under the different Zinc levels(0, 0.25, 0.50, 1.00, 2.00 mmol/L) in ryegrass(Lolium perenne L.). The results showed that low Zinc stress did not inhibit the growth of plants, but the shoot dry weights decreased under excessive Zinc stress(Zn≥2 mmol/L). The free proline content in shoot increased with the increase of time and Zinc level. The changes of POD activity and root system vigor were the parabola trend with the increase of time. The activity of POD in shoot decreased first, and then increased with the increase of Zinc level under Zinc treatment, while the root system vigor increased with the increase of Zinc level. The Zn content in the shoot and root increased with the increase of Zn level. The maximum of shoot Zn contents was 775.0 mg/kg(at 2.0 mmol/L Zn treatment).