Hydrogen production of heat treatment sludge, acid treatment sludge, normal sludge, base treatment sludge and ultrasonic sludge from simulated wastewater by anaerobic sewage were investigated. The substrate containing glucose was treated with anaerobic heat treatment sewage sludge. The biohydrogen was produced through anaerobic hydrogen fermentation. The effects of temperature, pH value and glucose concentration on hydrogen production were also investigated. The optimal temperature, pH value and concentration of glucose were 45℃, 6.0 and 5 g/L, respectively.