In order to solve the problem of poor water resistance of the starch-based adhesive after bonding wood materials, the impact of various factors on the wet strength was researched in the course of synthesizing adhesive with corn starch, polyvinyl alcohol, isocyanate and carboxylic styrene butadiene latex. The method of quadratic orthogonal rotation combination experiment design was applied, and SAS software and Matlab software were used to process data. Effects of the material and reaction temperature on wet strength of adhesive after bonding wood materials were analyzed to optimize technological parameters of starch-based adhesive The optimal conditions were as follows: starch content 13%, polyvinyl alcohol 4%, isocyanate content 5%, latex content 3%; reation temperature 58℃. The microstructure of starch-based adhesive was analyzed by scanning electron microscope to reveal the mechanism of the wet strength enhancement.