To further enhance the precision seedling productivity of super hybrid rice and seeding accuracy, the effects of working parameters (such as seeds thickness, double-sucker interval and vibration frequency) on seed suction performance were studied, taken a seeder device of air-suction cylinder seeder for super hybrid rice as test object. The regression model between seed suction performance and working parameters was obtained with three factors and five levels experiments by using computer vision technology and vibration analyzer. The results showed that seed-layer thickness was the most important performance index among the experimental factors under the conditions of vacuum pressure 3.1 kPa, rotate speed 14 r/min and vibration frequency among 90 and 104 Hz. The prime value of seed suction performance was 93.12% when the seed-layer thickness 7 mm, distance of double suckers 3 mm and frequency of vibration 97 Hz. The study provides a scientific basis for design and parameters optimization of air-suction cylinder seeder for super hybrid rice.