In order to reduce the risk of operators irradiated by the ultraviolet (UV) lamp in sterilization room, an intelligent monitoring system for ultraviolet sterilization room was designed based on Doppler shift. A biological recognition method by feature detection of human body on the move was presented, and the mathematical model of Doppler shift was established. The sample of biological recognition module was produced, and the experimental platform employing SPCE061A microcontroller units (MCU) were developed. The experiments of biological recognition were carried out. The experimental results show that the recognition probability of the intelligent monitoring system can reach 99.58%. This can meet the requirement in actual application.