Abstract: Hole-insertion grafting method is widely used for cucurbit crops. The method establishes a very strong graft union without grafting clips. The key of successful grafting is to make hole at an optimal angle in the removed bud region so that the edge where the tool comes out through hypocotyls was below the opposite cotyledon. So during the hole-insertion grafting, the insertion angle is an important factor for the quality and survival of grafting plants, and mechanical stability. This study aimed to assess common rootstock and scion grafting cultivation in pumpkin, cucumber and watermelon, and investigated the effect of the insertion angle on the grafting mechanize. To achieve the objectives, structure parameters of rootstock and scion were measured, internal structure of hypocotyl growing point was analyzed by image analysis technique. The model was developed. The maximal insertion angle (75°) was determined by the grafting requirement that the insertion should avoid damaging cotyledon and entering hypocotyl cavity.The results showed that the insertion angle of perforate stick was not more than 75° for avoiding the insertion into the hypocotyls cavity. The aim of this study was to develop the semi-automatic grafting mechanism for grafted seedling on the basis of the hole-insertion grafting method and the insertion angle, which the grafting mechanism was adjustable in the range of 45°-75°. The experiment was carried out for researching the insertion angle of pumpkin and cucumber seedling using the grafting mechanism. In this experiment, the insertion angles were respectively set at 45°, 50°, 55°, 60°, 62°, 65°, 70°. In this grafting processing, the rootstock seedling and scion seedling were placed respectively in the rootstock clamping device and scion clamping device by operator, the grafting mechanism automatically completed clamping, punching, cutting and inserting. This report investigated shaping position and matching between the scion and the rootstock base on the structure for grafted seedlings by using image analysis technique. In this grafting method, grafted cuttings will be rooted and inserted in a new tray containing well watered medium, and healed at the same time inside a healing chamber. It is important to keep grafting seedling in a high moisture environment during the formation of the grafting union and healing period. In this experiment, grafted seedlings were placed in 95% humidity, temperature 26-28℃ during day and 19-21℃ during night, and complete darkness for initial 3 days. After 3 days of darkness, light was added. The experiment results showed that the rate of scion inserted into the rootstock cavity was low with the insertion angle of 60°, the successful ratio of grafting mechanism was high, and grafting survival rate was 91%. This method was effective in the view of punching, cutting and grafting process.