Abstract: Biogas residue is a kind of high quality medium, being with comprehensive nutrition and rich in organic matter. In order to select new type of non-soil culture substrate, in this paper, the experiment for single factor randomized block design was carried out to study the effects of the mass fraction of biogas residue on physicochemical properties of compound organic substrate, growth characteristics of seedlings and grass turf traits of Festuca Arundinacea. In this experiment, biogas residue was used as raw material to compound organic substrate to plant tall fescue turf. The results were as follows: 1) For the T60 matrix (volume fraction of biogas residue was 60%), the total nitrogen, total phosphorus, total potassium, available nitrogen, available phosphorus, available potassium and organic matter content were the highest, and more than the other 3 matrices, which were CK, T30 and T90 (volume fractions of biogas residue were zero, 30% and 90%, respectively); with the increase of mass fraction of biogas residue in compound organic substrate, pH value of the compound matrix decreased significantly, both dry and wet bulk density decreased, both infiltration rate and total porosity increased, water retention increased, and this amelioration effect of biogas residue decreased with the increasing of mass fraction of biogas residue; 2) All 3 compound organic substrates had significant promoting effect on the seedling growth of F. Arundinacea. The seedling number per unit area growing on T90 substrate was 22242, 30616, and 31990 for the 10th, 20th, and 40th day after germination, which was increased by 54.77%, 92.01% and 91.09%, respectively, compared with CK. For the 10th day after germination, seedling height on T90 substrate was the highest, 86.36% higher than that on CK substrate. For the 20th day after germination, seedling heights on T60 and T90 substrate were the same, which were both 8 cm, 83.10% higher than that of seedling with CK substrate. In the 40th day after germination, seedling heights with T60 and T90 substrate were both 10 cm, 85.71% higher than that of seedling with CK substrate. Seedling leaf width on T90 substrate was respectively 1.34, 1.81 and 1.87cm for the 10th, 20th and 40th day after germination, and it respectively increased by 50.56%, 67.59% and 94.79% compared with that of seedling on CK substrate. From the point of view of seedling growth, both T60 and T90 substrate were suitable for the cultivation of tall fescue turf; 3) For the 10th day after germination, turf coverage on 3 compound organic substrates was higher than that of CK substrate; for the 20th and 40th day after germination, turf coverages with T60 substrate were respectively 81% and 100%, which were the highest and increased by 13.47% and 53.85% respectively compared with that of the CK substrate; the turf color and uniformity with T60 substrate was higher than that of the CK substrate; 4) For the 40th day after germination, total biomass, aboveground and underground biomass and root thickness of turf under T60 substrate were significantly greater than the other 3 substrates; root shoot ratio of turf was 3.94 on T60 substrate, which was only 28.10%, 14.53% and 7.94% of that under CK, T30 and T90 substrate, respectively. T60 substrate significantly changed the distribution of aboveground and underground biomass pattern of F. Arundinacea, and significantly promoted the biomass allocation to the aboveground. Therefore, T60 substrate was the most suitable compound organic substrate for the establishment of tall fescue turf.