解钾细菌与黏土矿物协同促进玉米生长提高土壤养分有效性
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国家自然科学基金(51574253);国家863计划项目(2013AA102904);内蒙古科技大学创新基金项目(2015XYPYL04)。


Synergistic effect of releasing potassium bacteria and clay minerals improving maize growth and soil nutrients availability
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    摘要:

    为了揭示解钾细菌在西北矿区浅埋古河道土壤中对植物生长和土壤养分利用的影响,通过日光温室短期盆栽的方式,以不同黏土矿物配比的人工培土为基质模拟古河道不同质地土壤,以西北地区常见农作物玉米为宿主,研究解钾细菌在人工培土基质中的微生物数量变化规律,以及二者协同作用对玉米生长和矿质养分的影响效果,,以及二者协同作用对玉米生长和土壤氮磷钾表观利用率的影响。结果表明:1)土壤黏土矿物含量增大有利于提高土壤解钾细菌数量,促进微生物活性。当黏土矿物质量分数为68%,速效钾质量分数约170 mg/kg时,解钾细菌数量最大;2)玉米地上部分干质量、根冠比、根系活力随黏土矿物含量增大而增大,以解钾细菌作用下黏土矿物质量分数68%的玉米生长效果最佳;3)在解钾细菌作用下,植物氮磷钾积累量和土壤养分利用的最佳土壤黏土矿物质量分数为45%、68%和75%,土壤钾素、氮素和磷素最大利用率分别达到65%、53%和17%;4)解钾细菌在土壤钾素含量低时促进土壤磷素吸收,土壤钾素过量时,促进土壤氮磷钾的吸收,提高土壤养分利用率。因此,土壤黏土矿物与解钾细菌相互作用,而且积极影响植物生长和土壤养分的吸收利用,这对进一步探寻适合矿区浅埋古河道土壤的微生物复垦技术,深入改良和开发矿区退化土壤具有重要意义。

    Abstract:

    Abstract: Widely distributed shallow-buried paleochannels sand-bodies are valuable reservoirs of shallow water resource in the coal mining area. It is located in the transition zone between Maowusu desert and Loess Plateau, in which ecological environment is fragile and vulnerable to damage. It is necessary to rationally develop and utilize soil in shallow-buried paleochannels for ecological restoration in the mining area of northwest china. In order to reveal the effects of releasing potassium bacteria on plant growth and soil nutrients utilization in the shallow-buried paleochannels in the Northwest Mining area, the study was performed by short-term pot cultures in heliogreenhouse with maize in artificial soils, which simulated the soils with different contents of clay minerals in paleochannels. Test artificial soils were composed of quartz and 2 kinds of potassium-rich clay minerals, including feldspar and illite which are common minerals in the soil of northwest mining area. The test artificial soils were treated with 6 levels of clay minerals, which followed by 18%, 25%, 38%, 45%, 68% and 75% mass fractions of clay minerals in soils. Each level of clay minerals treated with inoculation with active C6X or sterilized C6X. The C6X was the tested strains of releasing potassium bacteria Phyllobacterium ifriqiyense isolated from the garden soil in Beijing Suburbs by microbial reclamation laboratory in China University of Mining and Technology. We investigated the relationship between the releasing potassium bacteria quantity and the artificial soils with 6 different kinds of clay minerals ratios, and their synergistic effect on the growth of maize including dry weight of shoot, root shoot ratio and root activity, mineral nutrients of maize and apparent utilization ratios of nitrogen, phosphorus and potassium in soil. The results showed: 1) The quantity of releasing potassium bacteria was increased with increase in the clay minerals contents in soil. Releasing potassium bacteria quantity reached the peak value when the mass fraction of clay minerals in soil was 68% and concentration of exchangeable potassium in soil was 170 mg/kg, which was followed by decline of releasing potassium bacteria quantity at the mass fraction of clay minerals of 75%; 2) Maize dry weight of shoot, root shoot ratio, root activity were increased following the increase of clay minerals contents. When the mass fraction of clay minerals was 68% with inoculation with active C6X, the 3 indexes of maize growth reached maximum value; 3) The optimum mass fractions of clay minerals in soils with active C6X inoculation were 45%, 68% and 75% for accumulations of maize nitrogen, phosphorus and potassium and soil nutrients availability. The maximum apparent utilization ratios of soil potassium, nitrogen and phosphorus were 65%, 53% and 17%, respectively; 4) Releasing potassium bacteria promoted the absorption of phosphorus in the case of soil potassium deficiency (the mass fraction of clay minerals was 18%), while it promoted the absorption of nutrients in maize and use efficiency of nitrogen , phosphorus and potassium in the case that soil potassium exceeded (the mass fraction of clay minerals was 75%). The absorption and utilization of nitrogen, phosphorus and potassium in maize was closely associated with potassium content in the artificial soils, and was not significantly correlated with initial contents of nitrogen, phosphorus in the artificial soils. In sum, releasing potassium bacteria quantity was intensively influenced by the contents of clay minerals in soil. The interaction between clay minerals and releasing potassium bacteria in soil affected maize growth and nutrition uptake actively, also enhanced the utilization of mineral nutrients in soil. It provides useful information for exploring suitable microbial reclamation technology and improving degraded soil in mining area in northwest China.

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尚海丽,毕银丽,彭苏萍,解文武.解钾细菌与黏土矿物协同促进玉米生长提高土壤养分有效性[J].农业工程学报,2016,32(12):129-135. DOI:10.11975/j. issn.1002-6819.2016.12.019

Shang Haili, Bi Yinli, Peng Suping, Xie Wenwu. Synergistic effect of releasing potassium bacteria and clay minerals improving maize growth and soil nutrients availability[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE),2016,32(12):129-135. DOI:10.11975/j. issn.1002-6819.2016.12.019

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  • 收稿日期:2015-12-14
  • 最后修改日期:2016-04-10
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  • 在线发布日期: 2016-05-09
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