Coal is the primary energy source in china, large-scale coal mining causes serious ecological and environmental problems in China. For restoring farmland in such areas, reclaiming subsided land with Yellow River sediment is an effective reclamation technology. In this study, we investigated the influence of interlayer positions on soil water infiltration process of reclaimed soil filled with Yellow River sediment. The laboratory experiments of infiltration were conducted in the soil columns of 120 cm with 2 control treatments (CK1, CK2) and 5 interlayer treatments (T1-T5). CK1 consisted of 20-cm topsoil overlying subsoil, representing native undisturbed farmland. CK2 represented conventional reconstructed soil profile consisted of 20-cm topsoil and 40-cm subsoil overlying sediment and 60 cm Yellow River sediment on the bottom. Treatments T1-T5 represented reconstruct multi-layered soil profiles consisted of 20-cm topsoil, 20-cm subsoil and different combinations of sediment and 20-cm thick subsoil interlayer located at different position between the Yellow River sediment layer. In treatments of T1-T5, subsoil interlayer was filled into the column at the 50, 55, 60, 65, 70 cm away from the soil surface, respectively. The soil texture of subsoil was clay. Thus, the subsoil interlayer was also the clay interlayer. By the laboratory infiltration experiments, the influence of interlayer position on water infiltration was analyzed, and the subsoil interlayer position of the reclaimed soil filled with Yellow River sediment was optimized. Calculated index included infiltration rate, cumulative infiltration, depth to the wetting front, and water content volume distribution of the reclaimed soils. Finally, the filtration was fitted by Kostiakov model. The results showed that subsoil clay interlayer was more effective in inhibiting water leakage and improving the water-holding capacity of conventional reconstructed soil profile. The infiltration rate increased and then decreased with the increasing depth of subsoil interlayers with the same thickness. The position of the subsoil interlayer at 55 cm below the soil surface was a threshold where the infiltration rate was the lowest, the migration speed of the wetting front was the slowest, and the water resistance effect was the strongest. However, considering the heavy rainfall in the study area, surface runoff was easy to form. When the position of interlayer was 60 cm below the soil surface, the water infiltration characteristic was closer to that of undamaged farmland, and it was the optimal position of interlayer for land reclamation with Yellow River sediments. Moreover, the Kostiakov model could fit the infiltration process well for the reclaimed soil with clay interlayer in the Yellow River sediment layer (R2 was higher than 0.99 and relative root mean square error was not higher than 0.07). This study has important practical significance for extension of interlayer soil profile reclamations with Yellow River sediment.