In order to fully research the regional differences and dynamic evolution of cultivated land pressure to ensure food security in Hubei Province, the revised cultivated land pressure index was used to measure the pressure of cultivated land in Hubei Province and its 17 cities. The difference of cultivated land pressure variation in Hubei Province from time series and spatial level was analyzed. The kernel density estimation method was used to describe the dynamic evolution of cultivated land pressure. The results showed that: 1) During the period 2000-2016, the cultivated land pressure index and the minimum cultivated land area per capita showed a decrease trend. The average cultivated land pressure index was 1.011 in Hubei Province; the cultivated land pressure was warning pressure. The minimum per capita arable land area and per capita actual supply area were small. 2) During the period 2000-2016, the change of cultivated land pressure in Hubei Province is inconsistent. The temporal and spatial changes of cultivated land pressure were obvious, and the situation of cultivated land pressure was severe in the central area of Hubei Province, Wuhan in particular. The cultivated land pressure in western area was weaker than that of eastern area of Hubei Province, and the situation of cultivated land pressure in Xiangyang city was the best. The trend of cultivated land pressure in 17 urban areas was different, but the current situation of cultivated land pressure in Hubei was optimistic and the change was small. At the same time, the pressure of cultivated land in western and eastern Hubei provinces was different. In the eastern region, such as Wuhan, E'zhou and Huangshi, they had higher pressures on cultivated land, but Huanggang and Xianning had better pressure of cultivated land. Only the pressure of cultivated land in Xiangyang City reached the best. The pressure on cultivated land in the other four cities was higher than medium pressure; the annual pressure of cultivated land in Shiyan City was higher than high pressure. 3) The overall level of cultivated land pressure gradually decreased during the sample survey period, but the regional gap increased, and the polarization phenomenon was quite obvious. 4) From the perspective of different regions, the overall level of cultivated land pressure in the eastern Hubei was continuously decreasing, the regional gap was large, and the polarization phenomenon was more prominent; the pressure level of cultivated land in central Hubei was low, the regional gap was not large, and the polarization was not obvious; the pressure of cultivated land in western Hubei horizontally centered, there was a regional gap, and polarization was not obvious as the eastern region.