Abstract: A high demand has been growing for non-destructive, cost-saving, and online visual detection on the mutton freshness. In this study, a prepared smart label was used to detect the freshness of mutton stored at a commercially available temperature (10±2)℃, and then a reliable prediction model was established. This smart label was made of a nanofiber membrane fabricated by electrospinning, which was loaded with pH-sensitive anthocyanin and biodegradable polylactic acid. A scanning electron microscope (SEM) and UV-2450 spectroscopy were used to observe and detect the microstructure and amine sensitivity of the fiber membrane. Some parameters were measured during the storage of mutton, including the sensory quality, total volatile base nitrogen (TVB-N), pH value, the total number of colonies, acidity/oxidation capacity coefficient of meat, and fiber membrane color difference. The freshness of mutton was accurately graded, thereby preliminarily evaluating the accuracy of the fiber membrane for the detection. The results showed that the fiber membrane was amine sensitive in the light pink with uniform fiber filaments of about 250 nm. The freshness indicators of mutton demonstrated that it had been putrefied and deteriorated after 72 h storage, where the fiber membrane changed from pink to white. Correlation analysis showed that the color difference of the fiber membrane had a significant correlation with each freshness index. A prediction model was successfully established for the TVB-N with an accuracy rate of 88.2% was successfully established(R2=0.967). Therefore, anthocyanin nanofiber smart label can be preliminarily applied to the detection of mutton freshness, where the freshness could be roughly graded according to the color of the label. The reason was that the amines were produced during meat spoilage, some of which evaporated into the packaging environment, thereby changing the pH value of the environment, whereas, the anthocyanins were color responsive to pH, which caused the label color to change in turn. The price of the label was relatively low and the overall price was only 0.0027 Yuan, indicating a great advantage in market applications. The anthocyanin smart label can be used to realize the non-destructive, visual, and real-time detection of mutton freshness, providing a new idea to solve meat safety.