Soil and water conservation is one of the most important parts of the national ecological civilization. The spatial control area of soil and water conservation can be divided to effectively manage the soil and water loss regions. However, it is still lacking in the spatial delineation in the regional division of soil and water conservation. Only a few studies have been focused on the spatial control of soil and water conservation, according to the small watershed. This study aims to explore the spatial control zoning for soil and water conservation, and then implement the differentiated protection and management measures. The universal soil loss equation (USLE) model was used to calculate the potential and actual soil erosion. The main influencing factors of soil erosion were determined by random forests. A self-organizing feature map (SOFM) was used to determine the spatial control zone of soil and water conservation in the Dabie Mountain area on a small catchment scale. The results showed that: 1) The average potential and actual soil erosion were 84415.7 t/(km²·a) and 210.25 t/(km²·a), respectively. The actual soil erosion was distributed mainly in 0-300 t/(km²·a) at the small watershed scale. There was the basically same distribution of spatial patterns under the potential and actual soil erosion. The high-value area was distributed mainly in the central and eastern mountain areas at the high elevation. 2) Vegetation coverage and slope were the main influencing factors of potential and actual soil erosion at the small watershed scale, indicating a significantly positive correlation with the potential soil erosion (P<0.01). The high vegetation cover area was concentrated in the hinterland of Dabie Mountain. The high slope area was extended from the west to the east along the ridgeline of Dabie Mountain. 3) The SOFM results showed that the spatial control zone of soil and water conservation was divided into three areas: key prevention, general prevention, and the rest area at the small watershed scale. Among them, the key prevention area involved 710 small watersheds with an area of 1 5287.4 km². There were 890 small watersheds in the general prevention area, covering an area of 18 874.4 km². Two prevention areas accounted for 61.2% of the study area. There was an outstanding difference between actual and potential soil erosion and slope among regions. The classification index can serve as the spatial control of soil and water conservation. The finding can provide theoretical support and decision-making on the spatial control regionalization for soil and water conservation.